Title :
Development and Characterization of Choline and L-Glutamate Biosensor Integrated on Silicon Microprobes for In-Vivo Monitoring
Author :
Frey, O. ; van der Wal, P. ; de Rooij, N. ; Koudelka-Hep, M.
Author_Institution :
Univ. of Neuchatel, Neuchatel
Abstract :
A silicon microprobe to measure in real-time choline and L-glutamate in brain tissue is described. The biosensors are prepared by coating integrated platinum thin-film electrodes with an enzymatic membrane by electrochemically aided deposition. This method allows a very local and reproducible immobilization of the enzymes and the achievement of high sensitivities as required for in vivo recordings. Moreover, several closely-spaced electrode sites on the same microprobe can be coated with different enzyme membranes without any cross-contamination.
Keywords :
amperometric sensors; biological tissues; biomembranes; biosensors; brain; electrochemical electrodes; electrodeposition; enzymes; platinum; silicon; L-glutamate; Pt; Si; amperometric detection; biosensors; brain tissue; choline; electrochemically aided deposition; enzymatic membrane; enzyme immobilization; platinum thin-film electrodes; silicon microprobe; Biochemistry; Biomembranes; Biosensors; Brain; Coatings; Electrodes; Monitoring; Platinum; Silicon; Sputtering; Amperometric detection; Biosensor; Choline; L-Glutamate; Silicon microprobe; Alcohol Oxidoreductases; Amino Acid Oxidoreductases; Biosensing Techniques; Choline; Electrochemistry; Equipment Design; Equipment Failure Analysis; Glutamic Acid; Microelectrodes; Miniaturization; Reproducibility of Results; Sensitivity and Specificity; Silicon; Systems Integration;
Conference_Titel :
Engineering in Medicine and Biology Society, 2007. EMBS 2007. 29th Annual International Conference of the IEEE
Conference_Location :
Lyon
Print_ISBN :
978-1-4244-0787-3
DOI :
10.1109/IEMBS.2007.4353725
