Title :
Thermal analysis of peptides with a calorimeterchip
Author :
Iervolino, E. ; Finoulst, I.M.O. ; van Herwaarden, A.W. ; Wien, W. ; Verhaert, P.D.E.M. ; Sarro, P.M.
Author_Institution :
Xensor Integration, Delfgauw, Netherlands
Abstract :
In this paper we describe a simple method to measure the thermal conductivity and thermal diffusivity of three related biochemicals: an amino acid, a peptide and a protein in aqueous solution using a liquid calorimeter chip. The measurements are performed by applying an AC voltage to an integrated heater at 10 mHz and 1 Hz and measuring the output voltage of the thermopile. The biochemicals solution tested included: L-lysine dissolved in water at different concentrations (50; 10; 1; 0.1 wt%); alpha-mating factor dissolved in water at 0.1 wt% and the bovine serum albumin (BSA) at 1 wt%.
Keywords :
biochemistry; biological techniques; biothermics; calorimeters; lab-on-a-chip; molecular biophysics; proteins; thermal conductivity measurement; thermal diffusivity; AC voltage; L-lysine; amino acid; biochemical; frequency 1 Hz; frequency 10 mHz; integrated heater; liquid calorimeter chip; peptide; protein; thermal conductivity; thermal diffusivity; thermopile; Amino acids; Bovine; Conductivity measurement; Peptides; Performance evaluation; Proteins; Semiconductor device measurement; Testing; Thermal conductivity; Voltage measurement; Liquid calorimeter chip; protein; thermal properties;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International
Conference_Location :
Denver, CO
Print_ISBN :
978-1-4244-4190-7
Electronic_ISBN :
978-1-4244-4193-8
DOI :
10.1109/SENSOR.2009.5285550