Two-phase mini-separator to separate alpha-fetoProtein from fibrinogen and Immunoglobulin

This paper reports a two-phase mini-column separator that depletes immunoglobulin G (IgG) and separates alpha-fetoProtein (AFP) and fibrinogen. A mixture of IgG, fibrinogen, and AFP goes through the two-phase separator, where IgG is depleted by an affinity mini-column using Protein A/G agarose beads in the first phase. In the second phase, AFP is separated from fibrinogen by size exclusion chromatography (SEC). The IgG depletion and SEC separation results are confirmed by evaluating fluorescent intensity of the eluents. Red (IgG), green (AFP), and blue (fibrinogen) intensities show that over 99.5% of IgG is depleted and, in 10 min, the second phase SEC mini-column separates AFP (1 mug/mL) from fibrinogen-rich solution (10 mug/mL) with 2.78 resolution and 0.0056 mm plate height.