A microchip for automated extraction of RNA from Gram-positive bacteria

Author

Vulto, P. ; Hermann, C. ; Zahn, P. ; Maier, U. ; Dame, G. ; Urban, G.A.

Author_Institution

Dept. for Microsyst. Eng. (IMTEK), Albert-Ludwigs-Univ. Freiburg, Freiburg, Germany

fYear

2009

fDate

21-25 June 2009

Firstpage

108

Lastpage

111

Abstract

A Lab-on-a-Chip for fully automated quantitative extraction of RNA from Gram-positive bacterial cells is presented. The method combines thermo-electric lysis with electrophoretic purification. The chip is capable of RNA extraction proportional to the deployed amount of cells down to 1 colony forming units (CFU). The performance of the chip is demonstrated for transfer-messenger RNA (tmRNA) from the Gram-positive bacterium Streptococcus thermophilus (S.thermophilus). The RNA extraction lasts 10.5 minutes and the RNA yield is higher than for commercially available extraction kits. The extraction is particularly efficient for low molecular weight RNAs and may find broad application in clinical diagnostics and drug discovery.

Keywords

bioMEMS; biological techniques; cellular biophysics; electrophoresis; lab-on-a-chip; macromolecules; microorganisms; molecular biophysics; organic compounds; Gram positive bacteria RNA; Streptococcus thermophilus; automated quantitative RNA extraction; electrophoretic purification; lab on a chip; microchip; thermoelectric lysis; time 10.5 min; tmRNA; transfer messenger RNA; Biochemistry; Degradation; Electrodes; Isolation technology; Lab-on-a-chip; Microfluidics; Microorganisms; Purification; RNA; Wafer bonding; Ordyl; RNA extraction; bubble expulsion; electrophoretic purification; phaseguides; thermo-electric lysis;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International

Conference_Location

Denver, CO

Print_ISBN

978-1-4244-4190-7

Electronic_ISBN

978-1-4244-4193-8

Type

conf

DOI

10.1109/SENSOR.2009.5285553

Filename

5285553