A polymer cell chip with in situ monitoring capability of cell viability for cadmium cytotoxicity test of human rhabdomyosarcoma cells

This paper presents a polymer cell chip integrated with interdigitated array electrodes (IDA) and a planar cadmium (Cd) micro sensor for on-chip monitoring of cell culture and performing Cd cytotoxicity test of human rhabdomyosarcoma (HR) cells. HR cells were maintained in static culture on the cyclic olefin copolymer (COC) chip for up to seven days, and the cellular behavior was characterized using IDA by electrochemical impedance spectroscopy. A microfabricated Cd sensor with bismuth working electrode was adapted for in situ Cd sensing when cells were exposed to Cd at different concentrations, and the corresponding Cd cytotoxicity was monitored.