Title :
A Comparative Study of Adaptive Registration Methods for Two Dimensional Gel Electrophoresis Images
Author :
Huamei Xin ; Yuemin Zhu ; Longfei Wu
Author_Institution :
CNRS, Villeurbanne
Abstract :
Two-dimensional gel electrophoresis (2DGE) images play a major role in techniques for protein separation. The registration of 2DGE images is considered as one of key elements in protein identification. This paper proposes a single adaptive registration scheme for 2DGE images. Within the scheme, three registration methods based on the combined use of global linear affine and local nonlinear thin-plate spline (TPS), Demons, B-spline algorithms are explored and compared. The results show that the method using multi-resolution B-spline is preferred.
Keywords :
biochemistry; biological techniques; biology computing; electrophoresis; image registration; molecular biophysics; proteins; splines (mathematics); Demons; adaptive registration methods; global linear affine algorithm; image registration; multiresolution B-spline algorithms; nonlinear thin-plate spline algorithm; protein identification; protein separation; two dimensional gel electrophoresis images; Biological systems; Biomedical imaging; Electrokinetics; Image analysis; Image processing; Proteins; Proteomics; Spline; Two dimensional displays; Visualization; Algorithms; Artificial Intelligence; Electrophoresis, Gel, Two-Dimensional; Image Enhancement; Image Interpretation, Computer-Assisted; Pattern Recognition, Automated; Reproducibility of Results; Sensitivity and Specificity; Subtraction Technique;
Conference_Titel :
Engineering in Medicine and Biology Society, 2007. EMBS 2007. 29th Annual International Conference of the IEEE
Conference_Location :
Lyon
Print_ISBN :
978-1-4244-0787-3
DOI :
10.1109/IEMBS.2007.4353781
