Continuous multi-particle separation using deterministic focusing in spiral microchannels

This paper reports on a passive, high throughput approach to simultaneously separate multiple microparticles using the principle of Dean-coupled inertial migration in spiral microchannels. The design takes advantage of the particle size dependence of the inertial lift and Dean drag forces to equilibrate particles of varying sizes at distinct positions across the microchannel cross-section. The segregated particle streams are collected using an appropriately designed outlet system. The approach was demonstrated using a 5-loop 500 mum times 130 mum (W times H) Archimedean spiral channel to simultaneously separate 10 mum, 15 mum, and 20 mum diameter particles.