Electrically induced bubble knife

We have successfully operated enucleation of oocyte by using micro-electric knife without any thermal collateral damage. Minimally-invasive cellular-scale ablation was achieved by the mono-dispersed micro-bubbles which were generated by a pulse discharge of micro-electrode. The discharged output power and conductive area of micro-electrode were controlled by glass shell insulation around the copper micro-wire. A small space which is called “bubble reservoir” between the wire and glass tip contributes to stabilize the electric discharge and directional bubble generation.