DocumentCode
1858786
Title
Study of electroinjection of genes in cell under electrical fields
Author
Qingquan, Lei ; Guangming, Luo ; Xiaolin, Sun ; Xiaohung, Zhang
Author_Institution
Harbin Inst. of Electr. Technol., China
fYear
1994
fDate
23-26 Oct 1994
Firstpage
687
Lastpage
692
Abstract
Electroinjection of foreign genes into freely suspended cells was based on the reversible electrical breakdown of the cell membrane under an electrical field. It leads to the controllable, reversible permeability of the normally almost impermeable cell membrane. Here, we have studied the effect of the electrical parameters, cell and DNA concentration transformation efficiency, by comparing the experimental results, and obtained a set of optimum parameters. We have also given a reasonable interpretation of these results
Keywords
genetics; DNA concentration transformation efficiency; cell membrane; controllable reversible permeability; electrical fields; electrical parameters; electroinjection; foreign genes; freely suspended cells; genetic engineering; optimum parameters; reversible electrical breakdown; Biomembranes; Cells (biology); Chemical engineering; Chemical technology; DNA; Dielectrics; Dielectrophoresis; Genetic engineering; Sun; Voltage;
fLanguage
English
Publisher
ieee
Conference_Titel
Electrical Insulation and Dielectric Phenomena, 1994., IEEE 1994 Annual Report., Conference on
Conference_Location
Arlington, TX
Print_ISBN
0-7803-1950-8
Type
conf
DOI
10.1109/CEIDP.1994.592049
Filename
592049
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