Title :
Removing mesenchymal cells from gland tissue on micro-patterned tissue culture dish
Author :
Matsumoto, Tad ; Aoyagi, Shigehisa
Author_Institution :
Dept. of Biomater., Okayama Univ., Okayama, Japan
Abstract :
The simple and safety method for removing mesenchymal tissue from the extracted submandibular gland (SMG) tissue is required for the in vitro study of SMG tissue development. For this purpose, we fabricated polydimethylsiloxane (PDMS) tissue culture substrate having micropillar structure. By applying O2 plasma treatment and fibronectin coating, the surface affinity of PDMS substrate for cell and tissue was improved. Moreover, the pillar structure showed more effective to remove the mesenchymal tissue from isolated SMG tissue. Thus, the micro-fabricated surface with suitable chemical and physical modification is effective to remove mesenchymal tissue from extracted SMG tissue.
Keywords :
biochemistry; biological tissues; biomedical materials; cellular biophysics; microfabrication; molecular biophysics; polymers; proteins; tissue engineering; O2 plasma treatment; chemical modification; extracted submandibular gland tissue; fibronectin coating; mesenchymal cells; microfabricated surface; micropatterned tissue culture dish; micropillar structure; physical modification; polydimethylsiloxane tissue culture substrate; surface affinity; tissue development;
Conference_Titel :
Micro-NanoMechatronics and Human Science (MHS), 2012 International Symposium on
Conference_Location :
Nagoya
Print_ISBN :
978-1-4673-4811-9
DOI :
10.1109/MHS.2012.6492441
