Title :
Droplet formation and fusion for enzyme activity measurement by liquid dielectrophoresis
Author :
Kumemura, M. ; Yoshizawa, S. ; Collard, D. ; Fujita, H.
Author_Institution :
LIMMS-CNRS/IIS, Univ. of Tokyo, Tokyo, Japan
Abstract :
This paper describes a microdevice which is able to form and fuse 200 pL droplets containing biological materials in open environment by dielectrophoretic liquid actuation. To date, it is the smallest quantity manipulated by droplet in open space. The performance of this microdevice was confirmed by an enzymatic reaction validated by fluorescence.
Keywords :
bioMEMS; biochemistry; drops; electric actuators; electrophoresis; enzymes; fluorescence; microfluidics; molecular biophysics; wetting; biological materials; dielectrophoretic liquid actuation; droplet; enzyme activity measurement; fluorescence; microdevice; Biochemistry; Dielectrophoresis; Electrodes; Fabrication; Fluorescence; Frequency; Indium tin oxide; Microfluidics; Resists; Voltage; Droplet manipulation; Enzyme reaction; Liquid dielectrophoresis; Open environment;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International
Conference_Location :
Denver, CO
Print_ISBN :
978-1-4244-4190-7
Electronic_ISBN :
978-1-4244-4193-8
DOI :
10.1109/SENSOR.2009.5285715
