Title :
Repeatedly usable bio-molecule cantilever sensors with a coordination-bond breaking and re-linking technique
Author :
Yu, Haitao ; Li, Jungang ; Gan, Xiaohua ; Liu, Min ; Xu, Pengcheng ; Li, Xinxin
Author_Institution :
State Key Lab. of Transducer Technol., Chinese Acad. of Sci., Shanghai, China
Abstract :
This paper reports a method for recycled immobilizing and erasing antibody at micro-cantilever surface by a coordination-bond breaking and re-linking technique. With the new method, the sensor can be re-used to immuno-detect bio-molecules for many times. By our sensing experiment to killed vaccine of Bacillus Anthracis (BA) spores with concentration of 2000 spores/ml for three times, the target loaded on the cantilever surface causes a decrease of its frequency shift by 106 Hz, 100 Hz and 107 Hz, respectively. The biosensor detection sensitivity shows no decrease after multiple sensing cycles.
Keywords :
biosensors; cantilevers; microorganisms; microsensors; molecular biophysics; Bacillus anthracis spores; antibody; biosensor; coordination-bond breaking; coordination-bond relinking; detection sensitivity; immunodetection; microcantilever surface; recycled immobilizing; reusable biomolecule cantilever sensors; vaccine; Biosensors; Fabrication; Fungi; Micromachining; Protection; Resistors; Resonance; Silicon on insulator technology; Transducers; Vaccines; Bacillus Anthracis (BA) spores; Micro-cantilever; antibody; biosensor; recycled immobilizing;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International
Conference_Location :
Denver, CO
Print_ISBN :
978-1-4244-4190-7
Electronic_ISBN :
978-1-4244-4193-8
DOI :
10.1109/SENSOR.2009.5285722
