Computational model of DIC microscopy for reconstructing 3-D specimens: from observations to measurements

Author

Kagalwala, F. ; Kanade, Takeo ; Lanni, F.

Author_Institution

Robotics Inst., Carnegie Mellon Univ., Pittsburgh, PA, USA

fYear

1999

fDate

28-28 May 1999

Firstpage

80

Lastpage

81

Abstract

Summary form only given. Differential interference contrast (DIC) microscopy, a method pioneered by Georges Nomarski, is widely used to study live biological specimens. However, to date, biologists only qualitatively interpret DIC microscope images. In this work, we describe a method to extract quantitative information from optically-sectioned DIC microscope images. Specifically, given a set of images of a specimen, we attempt to reconstruct the three-dimension structure and refractive index distribution throughout the specimen.

Keywords

aberrations; biological techniques; image reconstruction; light interference; optical images; optical microscopy; ray tracing; refractive index; 3D structure reconstruction; Nomarski microscopy; aberrations; computational model; differential interference contrast microscopy; generalised ray tracer; interference image; iterative nonlinear optimisation; live biological specimen; optically sectioned microscope images; prism parameters; quantitative information; refractive index distribution; Biological system modeling; Biology computing; Biomedical optical imaging; Computational modeling; Data mining; Image reconstruction; Interference; Microscopy; Optical refraction; Optical variables control;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics, 1999. CLEO '99. Summaries of Papers Presented at the Conference on

Conference_Location

Baltimore, MD, USA

Print_ISBN

1-55752-595-1

Type

conf

DOI

10.1109/CLEO.1999.833909

Filename

833909