A fully addressable micro-array chip integrated with cascade multiplexors for selective cell loading and retrieval

We report a fully addressable micro-array chip integrated with cascade multiplexors for selective cell loading and retrieval in a stacked PDMS (polydimethylsiloxane) layers for microfluidic control. This chip provides the addressable manipulation of large number of cells at single cell resolution. A cascade multiplexer is developed to reduce the number of pneumatic control inputs integrated on chip. Hydrodynamic structures are incorporated for efficient single cell loading as well as retrieval. We have fabricated the prototype device in an 8 times 8 array and demonstrated the feasibility of single cell manipulation. We have loaded and cultured C2C12 cells in the prototype chip and then successfully retrieved the viable cells.