Title :
Improvement of detection sensitivity using electrodes with micropillar structures
Author :
Ginoza, T. ; Okamura, K. ; Fukuda, J. ; Suzuki, H.
Author_Institution :
Grad. Sch. of Pure & Appl. Sci., Univ. of Tsukuba, Tsukuba, Japan
Abstract :
Electrochemical biosensing has been generally considered to be highly efficient. However three-dimensional numerical analysis has revealed that only a fraction of an analyte is consumed for amperometric detection in a flowing stream. To improve the collection efficiency and the sensitivity of detection, micropillar electrodes were fabricated. Furthermore, a gold black layer was formed on the surface of the micropillars. In the best case, a 22-fold increase was observed in the generated current compared with a flat electrode. The micropillar electrodes were also used to conduct an electrochemical enzyme linked-immunosorbent assay.
Keywords :
amperometric sensors; bioMEMS; biosensors; computational fluid dynamics; electrochemical electrodes; enzymes; gold; 3D numerical analysis; amperometric detection; electrochemical biosensing; electrochemical enzyme linked-immunosorbent assay; electrodes detection sensitivity; micropillar electrodes; micropillar structures; Biosensors; Electrodes; Electrons; Glass; Gold; Immune system; Insulation; Sputtering; Substrates; Testing; Amperometry; Bone metabolic marker; Enzyme-linked immunosorbent assay; Flow channel; Gold black; Micropillars;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International
Conference_Location :
Denver, CO
Print_ISBN :
978-1-4244-4190-7
Electronic_ISBN :
978-1-4244-4193-8
DOI :
10.1109/SENSOR.2009.5285886
