Title :
Local stimulation to single cell on tissue-mimicking microfluidic device
Author :
Terao, K. ; Okonogi, A. ; Okitsu, T. ; Suzuki, T. ; Washizu, M. ; Kotera, H.
Author_Institution :
Dept. Micro Eng., Kyoto Univ., Kyoto, Japan
Abstract :
In this paper, we developed a novel device for the analysis of intracellular response to external stimulus in single-cell level with high-spatial resolution that simulates in vivo tissue micro-environment. We utilized multidirectional UV lithography technique to realize simple 3-dimensional fabrication of the tissue-mimicking device. Microchannel with an aperture at its sidewall was fabricated. Localized delivery of stimuli to single cell was demonstrated here.
Keywords :
bioMEMS; biological tissues; cellular biophysics; microchannel flow; microfabrication; ultraviolet lithography; 3-dimensional fabrication; external cell stimulus; high-spatial resolution; in vivo tissue micro-environment; intracellular response; microchannel flow; multidirectional UV lithography technique; single cell local stimulation; tissue-mimicking microfluidic device; Apertures; Cells (biology); Fabrication; In vivo; Lithography; Microchannel; Microfluidics; Optical microscopy; Pancreas; Sugar; Intracellular Imaging; Multi-directional UV Lithography; SU-8; Single Cell Analysis; Tissue Microenvironment;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International
Conference_Location :
Denver, CO
Print_ISBN :
978-1-4244-4190-7
Electronic_ISBN :
978-1-4244-4193-8
DOI :
10.1109/SENSOR.2009.5285887
