Optimising a Nitric oxide sensing technique for hypoxic tumor cell cultures

Author

Aravindalochanan, K. ; Kieninger, J. ; Sandvik, J.A. ; Pettersen, E.O. ; Urban, G.A.

Author_Institution

IMTEK, FRIAS Freiburg Inst. for Adv. Studies, Freiburg, Germany

fYear

2009

fDate

21-25 June 2009

Firstpage

1003

Lastpage

1006

Abstract

A nitric oxide (NO) electrochemical sensor for hypoxic, tumor cell culture applications is developed. Qualitative analysis of the electrode has been studied by cyclic voltammetry. NO amperometric measurement methods have been extensively analyzed. Square wave voltammetry method is found to be one of the optimal methods for the measurement of NO in cell cultures. A hypoxic tumor cell culture measurement (MCF-7 cell type) has also been conducted to observe the NO response to hypoxia.

Keywords

amperometric sensors; biomedical measurement; cancer; cellular biophysics; electrochemical electrodes; nitrogen compounds; tumours; voltammetry (chemical analysis); MCF-7 cell; NO; amperometric measurement method; cyclic voltammetry; electrochemical electrode; electrochemical sensor; hypoxic tumor cell cultures; nitric oxide sensing technique; qualitative analysis; square wave voltammetry method; Biosensors; Conductive films; Electrodes; Fabrication; Gold; Pollution measurement; Polymer films; Semiconductor device measurement; Sensor arrays; Tumors; Hypoxia; Nitric oxide; Square wave voltammetry;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International

Conference_Location

Denver, CO

Print_ISBN

978-1-4244-4190-7

Electronic_ISBN

978-1-4244-4193-8

Type

conf

DOI

10.1109/SENSOR.2009.5285957

Filename

5285957