A regenerative biosensing surface using electrochemical desorption of self-assembled monolayer in microfluidics

Author

Choi, Seokheun ; Chae, Junseok

Author_Institution

Dept. of Electr. Eng., Arizona State Univ., Tempe, AZ, USA

fYear

2009

fDate

21-25 June 2009

Firstpage

943

Lastpage

946

Abstract

We report a novel method to regenerate a biosensor surface in microfluidics. By applying a low DC voltage (0.9 V) between two electrodes, the sensing surface resets to be reusable/reconfigurable through electrodesorption of short-chain self assembled monolayers (SAMs). This method is free from electrolysis (HER (hydrogen evolution reaction)) and electrode oxidation (peel-off). Real-time SPR (surface plasmon resonance) measurements demonstrate streptavidin-bound COOH-SAM completely desorbs and CH₃-terminated SAM forms on the sensing surface to capture a target protein, fibrinogen, in a microfluidic device. The repeatability of the electrodesorption method is also presented.

Keywords

bioMEMS; biosensors; desorption; electrochemical electrodes; electrochemical sensors; microfluidics; microsensors; monolayers; proteins; self-assembly; surface plasmon resonance; DC voltage; electrochemical desorption; electrode oxidation; electrolysis; fibrinogen; microfluidics; protein; real-time SPR measurement; regenerative biosensing surface; self-assembled monolayer; streptavidin-bound COOH-SAM; surface plasmon resonance; voltage 0.9 V; Biosensors; Electrochemical processes; Electrodes; Hydrogen; Low voltage; Microfluidics; Oxidation; Plasmons; Proteins; Resonance; Biosensor; Electrochemical reductive desorption; Hydrogen Evolution Reaction (HER); Microfluidics; Reusable; Self-Assembled Monolayers (SAMs); electrode peel-off;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International

Conference_Location

Denver, CO

Print_ISBN

978-1-4244-4190-7

Electronic_ISBN

978-1-4244-4193-8

Type

conf

DOI

10.1109/SENSOR.2009.5285972

Filename

5285972