Title :
Continuous monitoring of insulin attachment kinetics on photothermally actuated microcantilever biosensor
Author :
Park, J.-W. ; Ducloux, O. ; Nishida, S. ; Fujita, H.
Author_Institution :
Inst. of Ind. Sci., CIRMM, Univ. of Tokyo, Tokyo, Japan
Abstract :
This study presents a method for monitoring kinetics of biological interaction using photothermally-actuated microcantilevers resonating in liquid. The surface of the cantilever was functionalized with anti-insulin-antibody to monitor insulin. By varying the insulin concentration, different reaction kinetics were observed in real time by monitoring the resonance frequency shift (Deltafr) of the MEMS resonator. We found that fast but weak absorption was dominant at high insulin concentration while slow but strong antigen-antibody binding is predominant at low insulin concentration.
Keywords :
bioMEMS; biochemistry; biosensors; cantilevers; enzymes; microsensors; molecular biophysics; photothermal effects; reaction kinetics; MEMS resonator; antigen-antibody binding; biological interaction; insulin attachment kinetics; insulin concentration; microcantilever biosensor; photothermal actuator; reaction kinetics; real-time anti-insulin-antibody; resonance frequency shift; Biosensors; Bonding; Etching; Insulin; Kinetic theory; Laser feedback; Laser modes; Monitoring; Resonance; Resonant frequency; Antibody; Insulin; Microcantilever; Photothermal laser; Reactive kinetics;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International
Conference_Location :
Denver, CO
Print_ISBN :
978-1-4244-4190-7
Electronic_ISBN :
978-1-4244-4193-8
DOI :
10.1109/SENSOR.2009.5285981
