Continuous monitoring of insulin attachment kinetics on photothermally actuated microcantilever biosensor

Author

Park, J.-W. ; Ducloux, O. ; Nishida, S. ; Fujita, H.

Author_Institution

Inst. of Ind. Sci., CIRMM, Univ. of Tokyo, Tokyo, Japan

fYear

2009

fDate

21-25 June 2009

Firstpage

979

Lastpage

982

Abstract

This study presents a method for monitoring kinetics of biological interaction using photothermally-actuated microcantilevers resonating in liquid. The surface of the cantilever was functionalized with anti-insulin-antibody to monitor insulin. By varying the insulin concentration, different reaction kinetics were observed in real time by monitoring the resonance frequency shift (Deltaf_r) of the MEMS resonator. We found that fast but weak absorption was dominant at high insulin concentration while slow but strong antigen-antibody binding is predominant at low insulin concentration.

Keywords

bioMEMS; biochemistry; biosensors; cantilevers; enzymes; microsensors; molecular biophysics; photothermal effects; reaction kinetics; MEMS resonator; antigen-antibody binding; biological interaction; insulin attachment kinetics; insulin concentration; microcantilever biosensor; photothermal actuator; reaction kinetics; real-time anti-insulin-antibody; resonance frequency shift; Biosensors; Bonding; Etching; Insulin; Kinetic theory; Laser feedback; Laser modes; Monitoring; Resonance; Resonant frequency; Antibody; Insulin; Microcantilever; Photothermal laser; Reactive kinetics;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009. International

Conference_Location

Denver, CO

Print_ISBN

978-1-4244-4190-7

Electronic_ISBN

978-1-4244-4193-8

Type

conf

DOI

10.1109/SENSOR.2009.5285981

Filename

5285981