DocumentCode
1867913
Title
Ultrasensitive detection of specific sequences in double-stranded DNA by laser-induced fluorescence
Author
Castro, A. ; Paz-Matos, L.
Author_Institution
Los Alamos Nat. Lab., NM, USA
fYear
1999
fDate
28-28 May 1999
Firstpage
254
Abstract
Summary form only given. Here, we describe a new method for the direct detection of specific sequences in double-stranded DNA. This method allows analysis of genomic samples without the need for a denaturing step. In addition, it can be combined with other single-molecule detection techniques that allow sizing DNA fragments by measuring their individual electrophoretic velocities. The present technique is based on a two-color, single fluorescent molecule detection technique.
Keywords
DNA; biomolecular effects of radiation; fluorescence; genetics; biological sample; biomedical diagnosis; double-stranded DNA; electrophoretic velocity; fragment sizing; gene detection; laser-induced fluorescence; sequence detection; single molecule detection; Aerosols; Atmospheric measurements; DNA; Diseases; Fluorescence; Laboratories; Microorganisms; Particle measurements; Probes; Sequences;
fLanguage
English
Publisher
ieee
Conference_Titel
Lasers and Electro-Optics, 1999. CLEO '99. Summaries of Papers Presented at the Conference on
Conference_Location
Baltimore, MD, USA
Print_ISBN
1-55752-595-1
Type
conf
DOI
10.1109/CLEO.1999.834152
Filename
834152
Link To Document