A fully monolithic microfluidic device for counting blood cells from raw blood

Author

Nguyen, John ; Yuan Wei ; Yi Zheng ; Chen Wang ; Yu Sun

Author_Institution

Univ. of Toronto, Toronto, ON, Canada

fYear

2015

fDate

18-22 Jan. 2015

Firstpage

435

Lastpage

438

Abstract

This paper reports a monolithic microfluidic device capable of complete blood constituent enumeration from raw blood. On-chip sample processing (e.g. dilution, lysis, and filtration) and downstream single cell measurement were fully integrated to enable sample preparation and single cell analysis from whole blood on a single device. The device consists of two parallel sub-systems that perform sample processing and electrical impedance measurements for measuring RBC and WBC parameters. Experimental characterization of patient blood samples validated the system´s capability for performing on-chip raw blood processing and measurement.

Keywords

biomedical measurement; cellular biophysics; electric impedance measurement; microchannel flow; blood cell counting; downstream single cell measurement; electrical impedance measurement; monolithic microfluidic device; on-chip raw blood measurement; on-chip raw blood processing; red blood cell parameter measurement; single cell analysis; white blood cell parameter measurement; Blood; Cells (biology); Electric variables measurement; Filtration; Microfluidics; Pollution measurement; System-on-chip;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems (MEMS), 2015 28th IEEE International Conference on

Conference_Location

Estoril

Type

conf

DOI

10.1109/MEMSYS.2015.7050983

Filename

7050983