• DocumentCode
    1871629
  • Title

    3D culture of mouse iPSCs in hydrogel core-shell microfibers

  • Author

    Ikeda, Kazuhiro ; Okitsu, Teru ; Onoe, Hiroaki ; Takeuchi, Shoji

  • Author_Institution
    Inst. of Ind. Sci., Univ. of Tokyo, Tokyo, Japan
  • fYear
    2015
  • fDate
    18-22 Jan. 2015
  • Firstpage
    463
  • Lastpage
    464
  • Abstract
    This paper reports the culturing and expansion of mouse induced pluripotent stem cells (iPSCs) in hydrogel core-shell microfibers; the core consists of iPSCs with or without extracellular matrix (ECM) proteins, and the shell is composed of calcium alginate. We revealed that mouse iPSCs cultured in the micro-scale space with ECM proteins sustain their pluriotency efficiently. This 3D culture system may be a useful tool to expand iPSCs for clinical use.
  • Keywords
    biomedical engineering; cellular biophysics; fibres; hydrogels; proteins; 3D mouse induced pluripotent stem cell culture; calcium alginate; extracellular matrix protein; hydrogel core-shell microfiber; mouse induced pluripotent stem cell expansion; pluriotency; Cells (biology); Educational institutions; Electronic countermeasures; Mice; Proteins; Stem cells; Three-dimensional displays;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    Micro Electro Mechanical Systems (MEMS), 2015 28th IEEE International Conference on
  • Conference_Location
    Estoril
  • Type

    conf

  • DOI
    10.1109/MEMSYS.2015.7050990
  • Filename
    7050990
    • Link To Document
    https://search.isc.ac/dl/search/defaultta.aspx?DTC=49&DC=1871629