Title :
3D culture of mouse iPSCs in hydrogel core-shell microfibers
Author :
Ikeda, Kazuhiro ; Okitsu, Teru ; Onoe, Hiroaki ; Takeuchi, Shoji
Author_Institution :
Inst. of Ind. Sci., Univ. of Tokyo, Tokyo, Japan
Abstract :
This paper reports the culturing and expansion of mouse induced pluripotent stem cells (iPSCs) in hydrogel core-shell microfibers; the core consists of iPSCs with or without extracellular matrix (ECM) proteins, and the shell is composed of calcium alginate. We revealed that mouse iPSCs cultured in the micro-scale space with ECM proteins sustain their pluriotency efficiently. This 3D culture system may be a useful tool to expand iPSCs for clinical use.
Keywords :
biomedical engineering; cellular biophysics; fibres; hydrogels; proteins; 3D mouse induced pluripotent stem cell culture; calcium alginate; extracellular matrix protein; hydrogel core-shell microfiber; mouse induced pluripotent stem cell expansion; pluriotency; Cells (biology); Educational institutions; Electronic countermeasures; Mice; Proteins; Stem cells; Three-dimensional displays;
Conference_Titel :
Micro Electro Mechanical Systems (MEMS), 2015 28th IEEE International Conference on
Conference_Location :
Estoril
DOI :
10.1109/MEMSYS.2015.7050990
