Alignment of collagen nanofibers in 2D substrates using cyclic stretch

Author

Eun Ryel Nam ; Won Chul Lee ; Takeuchi, Shoji

Author_Institution

Inst. of Ind. Sci., Univ. of Tokyo, Tokyo, Japan

fYear

2015

fDate

18-22 Jan. 2015

Firstpage

465

Lastpage

466

Abstract

This paper presents a new method to fabricate 2-dimensional collagen sheets whose internal molecular structures form highly-aligned nanofibers in one direction. In this work, collagen nanofibers are self-aligned in fully 2-dimensional substrates by applying cyclic stretch during the gelation process of collagen solution, and the fabricated collagen sheets induce the alignment of cells without any mechanical force within the cultivation period.

Keywords

biological specimen preparation; biomechanics; cellular biophysics; gels; materials preparation; molecular biophysics; molecular configurations; molecular orientation; nanobiotechnology; nanofabrication; nanofibres; natural fibres; proteins; sheet materials; substrates; 2-dimensional collagen sheet fabrication; 2D substrate; cell alignment; collagen nanofiber alignment; collagen nanofiber self-alignment; collagen solution; cultivation period; cyclic stretch application; gelation; highly-aligned nanofiber formation; internal molecular structure; mechanical force; Films; Force; In vitro; Microscopy; Nanobioscience; Optical fiber devices; Substrates;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems (MEMS), 2015 28th IEEE International Conference on

Conference_Location

Estoril

Type

conf

DOI

10.1109/MEMSYS.2015.7050991

Filename

7050991