Reconfigurable microfluidic dilution generation for quantitative assay

In this paper, we present a reconfigurable microfluidic dilution device for high-throughput quantitative assays, without the assistance of continuous fluidic pump or vacuum source. This device is capable to produce discrete logarithmic concentration profiles ranging from 1 to 100 fold dilution in parallel from a fixed sample volume (e.g., 10 μL). To facilitate mixing and reactions in the chambers, two acoustic microstreaming actuation mechanisms have been investigated for easy integratability and accessibility. We characterized the dilution performance by both colorimetric and fluorescent means.