Arrayed Monodisperse Micro-Alginate Beads in μ -Fluidic Traps for Cell Assay

We present two achievements in this paper: (i) the preparation of monodisperse Micro-Alginate Beads (MABs) with coefficient of variation (C.V.) less than 3% by combining T-junction droplet formation with internal gelation method, and (ii) the manipulation of flow resistances in passive μ-Fluidic traps to achieve “ one bead-to-one trap” immobilization for cell array. In addition, we have succeeded in encapsulating Hep G2 cells in MABs. Based on these results, we can develop a gentle and easy to handle bead-based cell assay system.