Automated Oxidase-Coupled Amperometric Microsensor with Integrated Electrochemical Actuation System for Continuous Sensing of Saccharoids

Recent developments for biosensors have been mainly focused on miniaturization and exploratory use of new materials. It should be emphasized that the absence of an "in-situ self-calibration/diagnosis technique" that is not connected to an external apparatus is a key obstacle to the realization of a biosensor for continuous use with minimum attendance. To address this deficiency, a needle-type biosensor system with fully automated operations is being developed, in which an oxidase-coupled amperometric sensor with oxygen depleting/generating actuator is interfaced with an electrochemical instrument and a perfusion system. Labview virtual instrument has been also developed to oversee the automatic control of the prototype sensor. Using the proposed system, a large amount of data can be rapidly collected for more effective sensor characterization and more advanced sensor designs. Autonomous and continuous sensing and self-calibration with minimal human intervention is also envisioned