Title :
4-D Hippocampus Measurements of Neurite Growth in vitro
Author :
Dempsey, K.P. ; Lam, K.P. ; Smith, W.A. ; Collins, D.J. ; Mazzocchi Jones, D.J. ; Yang, Yi ; Richardson, J.B.
Author_Institution :
Comput. Sci. & Math., EPSAM Univ. of Keele Staffordshire, Keele, UK
Abstract :
Current research in neural tissue engineering relies heavily on in vivo animal models of neurological injury. This has a number of major drawbacks. Using innovative imaging technologies and allied data analytics, this paper presents our current work on analysing advanced 3-D models of the hippocampal circuitry, using a laminar 3-D network of orthogonally orientated nanoscale fibres constructed within a 3-D collagen gel matrix. Preliminary work on primary animal neuronal cells was able to reveal the complex responses of the neuronal cells to chemical attachment factors and topographical cues present in the in vivo-like environment of the constructed 3-D scaffold. Results suggest that the approach has validity and has helped in defining important parameters for future investigations.
Keywords :
biomedical optical imaging; cellular biophysics; gels; injuries; molecular biophysics; nanomedicine; neurophysiology; proteins; tissue engineering; 3D collagen gel matrix; 4D hippocampus measurements; allied data analytics; animal neuronal cells; chemical attachment factors; constructed 3D scaffold; hippocampal circuitry; in vitro neurite growth; in vivo animal models; in vivo-like environment; laminar 3D network; neural tissue engineering; neurological injury; orthogonally orientated nanoscale fibres; topographical cues; Electronic countermeasures; Hippocampus; In vitro; Integrated circuit modeling; Microscopy; Solid modeling; 3-D Hippocampus Modelling; Focus depth estimators; Neurite outgrowth; Spatiotemporal/4-D Imaging & Analysis; Unlabelled continuous live cultures;
Conference_Titel :
Computer and Information Technology (CIT), 2014 IEEE International Conference on
Conference_Location :
Xi´an
DOI :
10.1109/CIT.2014.160