Title :
A novel microfluidic system for fluorescent sample analysis fabricated by rapid prototyping
Author :
Rosenauer, Michael ; Vellekoop, Michiel J.
Author_Institution :
Inst. of Sensor & Actuator Syst., Vienna Univ. of Technol, Vienna
Abstract :
In this paper we present the design, simulation and measurement results of a microfluidic optical sensor system for fluorescent sample analysis fabricated by single-layer micro-stereolithographic prototyping in a transparent photoresin. With rapid fabrication and simple packaging processes the device can be manufactured in less than one hour. The analysis efficiency of the sample observation zone is enhanced by a novel arrangement of the light focusing unit including integrated cylindrical lenses and inserted glass fibers. Using multiple angles for excitation and detection of the fluorescent signal the sensor allows simultaneous signal measurements and correlations to achieve a better S/N-ratio. The optical performance of the sensor chip is evaluated by measuring the light focusing efficiency and fluorescent signal intensities of different Rhodamine B stained sample flow concentrations.
Keywords :
flow sensors; fluorescence; integrated optics; microfluidics; optical fibres; optical sensors; Rhodamine B; fluorescent sample analysis; fluorescent signal intensities; integrated cylindrical lenses; light focusing efficiency; microfluidic optical sensor system; rapid prototyping; sensor chip; single-layer microstereolithographic prototyping; transparent photoresin; Analytical models; Fluorescence; Microfluidics; Optical design; Optical device fabrication; Optical sensors; Packaging; Prototypes; Semiconductor device measurement; Virtual prototyping;
Conference_Titel :
Sensors, 2008 IEEE
Conference_Location :
Lecce
Print_ISBN :
978-1-4244-2580-8
Electronic_ISBN :
1930-0395
DOI :
10.1109/ICSENS.2008.4716401