HPLC/ESI-MS identification of common peptides in amphibian skin secretions

Author

Zheng, Huiru ; Ojha, Piyush C. ; McClean, Stephen ; Robinson, Robert ; Black, Norman D. ; Hughes, John G. ; Shaw, Chris

Author_Institution

Fac. of Informatics, Ulster Univ., Jordanstown, UK

fYear

2003

fDate

24-26 April 2003

Firstpage

1

Lastpage

4

Abstract

Amphibian skin secretions are rich in peptides which are linked to defense mechanisms. Peptides that appear in the skin secretions of two or more species are likely to have desirable biological activity such as antibiotic, antimicrobial or analgesic properties. We present a computational protocol and associated algorithms for analysing low-mass-resolution high-performance liquid chromatography/electrospray ionization mass spectrometry (HPLC/ESI-MS) data from skin secretions and flagging candidate peptides for chemical sequencing. Results from analysis of Litoria Aurea, Litoria Caerulea and Litoria Infrafrenata are presented.

Keywords

biochemistry; biological techniques; biology computing; chromatography; mass spectroscopic chemical analysis; molecular biophysics; molecular weight; organic compounds; proteins; skin; HPLC/ESI-MS identification; Litoria Aurea; Litoria Caerulea; Litoria Infrafrenata; algorithms; amphibian skin secretions; analgesic properties; antibiotic properties; antimicrobial properties; candidate peptides; chemical sequencing; common peptides; computational protocol; defense mechanisms; desirable biological activity; low-mass-resolution high-performance liquid chromatography/electrospray ionization mass spectrometry; Algorithm design and analysis; Antibiotics; Biology computing; Chemical analysis; Fluids and secretions; Ionization; Mass spectroscopy; Peptides; Protocols; Skin;

fLanguage

English

Publisher

ieee

Conference_Titel

Information Technology Applications in Biomedicine, 2003. 4th International IEEE EMBS Special Topic Conference on

Print_ISBN

0-7803-7667-6

Type

conf

DOI

10.1109/ITAB.2003.1222397

Filename

1222397