Title :
Cantilever array sensor for multiple liver cancer biomarkers detection
Author :
Jingjing Wang ; Shuaipeng Wang ; Xing Wang ; Yinfang Zhu ; Jinling Yang ; Fuhua Yang
Author_Institution :
Inst. of Semicond., Beijing, China
Abstract :
A novel microcantilever biosensor was batch-fabricated with IC compatible MEMS technology for joint detection of liver cancer biomarkers with high sensitivity, high throughput, high specification, and good precision. A micro-cavity was designed in the free end of the cantilever for local antibody-immobilization using micro printing system, which can dramatically reduce the effect of adsorption-induced k variation. A linear relationship between the resonance frequency shift and the antigen concentration was observed for three liver cancer biomarkers, AFP, GGT-2, and HGF. In addition, the presented immunosensing method has little cross-reactivity to different antigen, paving the way to a highly specific technique. These approaches will promote clinical application of the cantilever sensors in cancer early diagnosis.
Keywords :
bioMEMS; cancer; lab-on-a-chip; liver; patient diagnosis; sensor arrays; AFP; GGT-2; HGF; IC compatible MEMS technology; antigen concentration; batch-fabrication; cancer early diagnosis; cantilever array sensor; immunosensing method; local antibody-immobilization; micro printing system; microcavity; multiple liver cancer biomarkers detection; resonance frequency shift; Arrays; Biomarkers; Biosensors; Cancer; Liver; Resonant frequency; Sensitivity; MEMS cantilever; liver cancer; mass detection; multiple biomarkers; stiffness coefficient k;
Conference_Titel :
SENSORS, 2014 IEEE
Conference_Location :
Valencia
DOI :
10.1109/ICSENS.2014.6985004
