Cantilever array sensor for multiple liver cancer biomarkers detection

Author

Jingjing Wang ; Shuaipeng Wang ; Xing Wang ; Yinfang Zhu ; Jinling Yang ; Fuhua Yang

Author_Institution

Inst. of Semicond., Beijing, China

fYear

2014

fDate

2-5 Nov. 2014

Firstpage

343

Lastpage

346

Abstract

A novel microcantilever biosensor was batch-fabricated with IC compatible MEMS technology for joint detection of liver cancer biomarkers with high sensitivity, high throughput, high specification, and good precision. A micro-cavity was designed in the free end of the cantilever for local antibody-immobilization using micro printing system, which can dramatically reduce the effect of adsorption-induced k variation. A linear relationship between the resonance frequency shift and the antigen concentration was observed for three liver cancer biomarkers, AFP, GGT-2, and HGF. In addition, the presented immunosensing method has little cross-reactivity to different antigen, paving the way to a highly specific technique. These approaches will promote clinical application of the cantilever sensors in cancer early diagnosis.

Keywords

bioMEMS; cancer; lab-on-a-chip; liver; patient diagnosis; sensor arrays; AFP; GGT-2; HGF; IC compatible MEMS technology; antigen concentration; batch-fabrication; cancer early diagnosis; cantilever array sensor; immunosensing method; local antibody-immobilization; micro printing system; microcavity; multiple liver cancer biomarkers detection; resonance frequency shift; Arrays; Biomarkers; Biosensors; Cancer; Liver; Resonant frequency; Sensitivity; MEMS cantilever; liver cancer; mass detection; multiple biomarkers; stiffness coefficient k;

fLanguage

English

Publisher

ieee

Conference_Titel

SENSORS, 2014 IEEE

Conference_Location

Valencia

Type

conf

DOI

10.1109/ICSENS.2014.6985004

Filename

6985004