DocumentCode
1895796
Title
Label free detection of PCR amplification
Author
Liu, Yi-Shao ; Banada, Padmapriya P. ; Bhunia, Arun K. ; Bashir, Rashid
Author_Institution
Micro & Nanotechnol. Lab., Univ. of Illinois at Urbana-Champaign, Urbana-Champaign, IL
fYear
2008
fDate
26-29 Oct. 2008
Firstpage
550
Lastpage
553
Abstract
In this paper, we present a method for the label-free detection of PCR products using impedance spectroscopy. Instead of using conventional optical detection instruments, our method provides a direct detection of amplified target genes in PCR. As a proof of concept, identification of a target bacterium in a mixture of microorganisms is demonstrated.
Keywords
microorganisms; spectroscopy; PCR amplification; impedance spectroscopy; label free detection; optical detection instruments; target bacterium; Biomedical engineering; Conductivity; Costs; DNA; Dielectrics; Electrokinetics; Impedance; Laboratories; Optical devices; Tellurium;
fLanguage
English
Publisher
ieee
Conference_Titel
Sensors, 2008 IEEE
Conference_Location
Lecce
ISSN
1930-0395
Print_ISBN
978-1-4244-2580-8
Electronic_ISBN
1930-0395
Type
conf
DOI
10.1109/ICSENS.2008.4716498
Filename
4716498
Link To Document