Title :
Label free detection of PCR amplification
Author :
Liu, Yi-Shao ; Banada, Padmapriya P. ; Bhunia, Arun K. ; Bashir, Rashid
Author_Institution :
Micro & Nanotechnol. Lab., Univ. of Illinois at Urbana-Champaign, Urbana-Champaign, IL
Abstract :
In this paper, we present a method for the label-free detection of PCR products using impedance spectroscopy. Instead of using conventional optical detection instruments, our method provides a direct detection of amplified target genes in PCR. As a proof of concept, identification of a target bacterium in a mixture of microorganisms is demonstrated.
Keywords :
microorganisms; spectroscopy; PCR amplification; impedance spectroscopy; label free detection; optical detection instruments; target bacterium; Biomedical engineering; Conductivity; Costs; DNA; Dielectrics; Electrokinetics; Impedance; Laboratories; Optical devices; Tellurium;
Conference_Titel :
Sensors, 2008 IEEE
Conference_Location :
Lecce
Print_ISBN :
978-1-4244-2580-8
Electronic_ISBN :
1930-0395
DOI :
10.1109/ICSENS.2008.4716498
