• DocumentCode
    1895796
  • Title

    Label free detection of PCR amplification

  • Author

    Liu, Yi-Shao ; Banada, Padmapriya P. ; Bhunia, Arun K. ; Bashir, Rashid

  • Author_Institution
    Micro & Nanotechnol. Lab., Univ. of Illinois at Urbana-Champaign, Urbana-Champaign, IL
  • fYear
    2008
  • fDate
    26-29 Oct. 2008
  • Firstpage
    550
  • Lastpage
    553
  • Abstract
    In this paper, we present a method for the label-free detection of PCR products using impedance spectroscopy. Instead of using conventional optical detection instruments, our method provides a direct detection of amplified target genes in PCR. As a proof of concept, identification of a target bacterium in a mixture of microorganisms is demonstrated.
  • Keywords
    microorganisms; spectroscopy; PCR amplification; impedance spectroscopy; label free detection; optical detection instruments; target bacterium; Biomedical engineering; Conductivity; Costs; DNA; Dielectrics; Electrokinetics; Impedance; Laboratories; Optical devices; Tellurium;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    Sensors, 2008 IEEE
  • Conference_Location
    Lecce
  • ISSN
    1930-0395
  • Print_ISBN
    978-1-4244-2580-8
  • Electronic_ISBN
    1930-0395
  • Type

    conf

  • DOI
    10.1109/ICSENS.2008.4716498
  • Filename
    4716498