DocumentCode :
190004
Title :
Biomarker quantification at clinically relevant concentrations using metal enhanced fluorescence combined with surface acoustic waves
Author :
Morrill, Samuel ; Bhethanabotla, Venkat ; Richardson, Mandek
Author_Institution :
Chem. & Biomed. Eng. Dept., Univ. of South Florida, Tampa, FL, USA
fYear :
2014
fDate :
2-5 Nov. 2014
Firstpage :
1483
Lastpage :
1486
Abstract :
Immunofluorescence assays are commonly used for the detection of proteins and antigens. The problems with these types of assays are that they suffer from weak fluorescence signals causing poor detection limits, background signals cause “noise” and falsepositives, and attachment times of antigens to antibody are fairly slow. In this work, metal-enhanced fluorescence was used to strengthen the fluorescence signal by a factor as large as 1000 and surface acoustic wave devices were used to remove background signals from non-specifically bound proteins and speed up the rate of attachment by a factor of six. These two were then combined to prove that these two phenomena can coexist.
Keywords :
biological techniques; fluorescence; proteins; surface acoustic waves; antigens; biomarker quantification; detection limits; false positives; immunofluorescence assays; metal enhanced fluorescence; noise; proteins; surface acoustic waves; Fluorescence; Immune system; Proteins; Silver; Surface acoustic wave devices; Surface acoustic waves; Immunofluorescence; Metal-Enhanced Fluorescence; Micro-mixing; Non-specifically bound proteins; Removal;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
SENSORS, 2014 IEEE
Conference_Location :
Valencia
Type :
conf
DOI :
10.1109/ICSENS.2014.6985295
Filename :
6985295
Link To Document :
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