Role of ADAR in determining the substitution pattern in sigma virus (Rhabdoviridae) in Drosophila melanogaster

Author

Piontkivska, Helen ; Paul, Sudipta ; Scharfenberg, Brian ; Wayne, Marta ; Matos, Luis F. ; Farmerie, William G. ; Miyamoto, Michael M.

Author_Institution

Dept. of Biol. Sci., Kent State Univ., Kent, OH, USA

fYear

2014

fDate

2-4 June 2014

Firstpage

1

Lastpage

1

Abstract

Host-mediated, selective RNA editing of adenosines to inosines (ADAR) can lead to hypermutation in some viruses. ADAR may contribute to the control of viral infection by preventing the transcripts from being transported into the cytoplasm. However, the precise role of ADAR and how prevalent it is among insect viruses is unclear. Previous PCR-based studies showed ADAR occurs but at low frequency in sigma virus (DMelSV), a virus of Drosophila melanogaster. Here we use SOLiD™ deep-sequencing from multiple individual flies within a single host population to comprehensively evaluate the patterns of sequence variation in sigma virus from within hosts to between host populations, and to determine how much, if any, of the observed variation can be attributed to ADAR action.

Keywords

RNA; biomembranes; cellular biophysics; microorganisms; molecular biophysics; ADAR action; ADAR role; Drosophila melanogaster; SOLiDTM deep-sequencing; adenosines; cytoplasm; hypermutation; inosines; selective RNA editing; sequence variation patterns; sigma virus; sigma virus rhabdoviridae; single host population; substitution pattern; viral infection; Bioinformatics; Educational institutions; Genomics; RNA; Sociology; Statistics; Viruses (medical); ADAR; Drosophila melanogaster; sigma rhabdovirus; substitution pattern;

fLanguage

English

Publisher

ieee

Conference_Titel

Computational Advances in Bio and Medical Sciences (ICCABS), 2014 IEEE 4th International Conference on

Conference_Location

Miami, FL

Print_ISBN

978-1-4799-5786-6

Type

conf

DOI

10.1109/ICCABS.2014.6863939

Filename

6863939