A Restoration and Extrapolation Iterative Method for Band-limited Fluorescence Microscopy Image

Author

Ponti-Junior, Moacir P. ; Mascarenhas, Nelson D A ; Suazo, Cláudio A T

Author_Institution

Univ. Fed. de Sao Carlos, Sao Carlos

fYear

2007

fDate

7-10 Oct. 2007

Firstpage

271

Lastpage

280

Abstract

Optical microscopic images, especially with a nonconfocal microscope, are fundamentally limited because the optical transfer function (the Fourier transform of the point-spread function) is zero over a region of the spatial-frequency domain. Iterative algorithms were developed for the restoration and extrapolation of diffraction-limited imagery. In this paper we present the effectiveness of an iterative method based on the Richardson-Lucy algorithm for image restoration and a simultaneous modified version of Gerchberg-Papoulis method to extrapolate the spectrum and control the noise amplification. Good convergence stabilization results were achieved and also good numerical results were observed.

Keywords

Fourier transforms; extrapolation; image restoration; iterative methods; optical microscopy; optical transfer function; Fourier transform; Gerchberg-Papoulis method; Richardson-Lucy algorithm; band-limited fluorescence microscopy image; diffraction-limited imagery; extrapolation; image restoration; iterative method; nonconfocal microscope; optical microscopic images; optical transfer function; point-spread function; spatial-frequency domain; Convergence of numerical methods; Extrapolation; Fluorescence; Fourier transforms; Image restoration; Iterative algorithms; Iterative methods; Optical diffraction; Optical microscopy; Transfer functions;

fLanguage

English

Publisher

ieee

Conference_Titel

Computer Graphics and Image Processing, 2007. SIBGRAPI 2007. XX Brazilian Symposium on

Conference_Location

Minas Gerais

ISSN

1530-1834

Print_ISBN

978-0-7695-2996-7

Type

conf

DOI

10.1109/SIBGRAPI.2007.25

Filename

4368194