Title :
Quantifying molecular colocalization in live cell fluorescence microscopy
Author :
Humpert, Fabian ; Yahiatene, Idir ; Lummer, Martina ; Sauer, Matthias ; Huser, T.
Author_Institution :
Biomol. Photonics, Bielefeld Univ., Bielefeld, Germany
Abstract :
One of the most challenging tasks in microscopy is the quantitative identification and characterization of molecular interactions. In living cells this task is typically performed by fluorescent labeling of the interaction partners with spectrally distinct fluorophores and imaging in different color channels. Potential interaction can then be determined by parameters that can measure the degree of molecular colocalization.
Keywords :
cellular biophysics; fluorescence; molecular biophysics; molecular configurations; optical microscopy; proteins; color channel imaging; fluorescent labeling; live cell fluorescence microscopy; molecular colocalization; molecular interactions; potential interaction; quantitative characterization; quantitative identification; spectrally distinct fluorophores; Colored noise; Correlation; Educational institutions; Image color analysis; Microscopy; Noise level; Signal to noise ratio;
Conference_Titel :
Lasers and Electro-Optics Europe (CLEO EUROPE/IQEC), 2013 Conference on and International Quantum Electronics Conference
Conference_Location :
Munich
Print_ISBN :
978-1-4799-0593-5
DOI :
10.1109/CLEOE-IQEC.2013.6801498