Imaging lipid films using polarization-sensitive third-harmonic generation

Author

Bautista, Godofredo ; Huttunen, Mikko J. ; Pfisterer, S. ; Kanerva, K. ; Ikonen, E. ; Kauranen, Martti

Author_Institution

Dept. of Phys., Tampere Univ. of Technol., Tampere, Finland

fYear

2013

fDate

12-16 May 2013

Firstpage

1

Lastpage

1

Abstract

This paper presents the application of polarization-sensitive THG microscopy to examine lipid structures. Significant variations in the THG signals across the lipid film under different tightly focused polarizations were observed, suggesting evidence of ordered regions in lipid structures. This work suggests that THG microscopy equipped with polarization-engineered beams can be used as feasible characterization probes for investigating the nonlinear optical properties of lipid structures.

Keywords

bio-optics; biological techniques; light polarisation; lipid bilayers; optical harmonic generation; optical microscopy; THG microscopy; characterization probes; lipid films imaging; lipid structures; nonlinear optical properties; ordered regions; polarization-engineered beams; polarization-sensitive third-harmonic generation microscopy; tightly focused polarizations; Anisotropic magnetoresistance; Educational institutions; Films; Lipidomics; Microscopy; Optimized production technology;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics Europe (CLEO EUROPE/IQEC), 2013 Conference on and International Quantum Electronics Conference

Conference_Location

Munich

Print_ISBN

978-1-4799-0593-5

Type

conf

DOI

10.1109/CLEOE-IQEC.2013.6801505

Filename

6801505