DocumentCode :
1930925
Title :
Effects of exposure to intermediate frequency magnetic fields on gene expression of estrogen-regulated gene in MCF-7 cells
Author :
Ogasawara, Yuki ; Ikehata, Masateru ; Sakaguchi, Ryo ; Awakura, Shiho ; Yoshie, Sachiko ; Ohkubo, Chiyoji ; Ishii, Kazuyuki
Author_Institution :
Meiji Pharm. Univ., Kiyose, Japan
fYear :
2011
fDate :
13-20 Aug. 2011
Firstpage :
1
Lastpage :
3
Abstract :
To evaluate biological effects of intermediate frequency magnetic fields (IF-MF), estrogen-regulated gene expression under magnetic fields were studied. Genetically modified MCF-7 cells that transformed with ERE-luc fusion gene was used. Cells which endogenous estrogen depleted by estrogen free media are exposed to 21 kHz IF-MF for 24 hr. Then, luciferase activity was measured as estrogen-regulated gene expression. In this study, we have observed no significant difference in luciferase activity between exposed and sham-exposed cells by exposure to up to 3.9mT, 21 kHz IF-MF for 24hr. These results suggest that IF-MF is unlikely to affect directly on estrogen-regulated gene expression.
Keywords :
biological effects of fields; biomagnetism; cellular effects of radiation; enzymes; genetics; ERE-luc fusion gene; MCF-7 cells; estrogen-regulated gene; frequency 21 kHz; gene expression; intermediate frequency magnetic field effect; luciferase activity; time 24 hr; Fetus; Gene expression; Magnetic fields; Media; Pregnancy; Proteins;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
General Assembly and Scientific Symposium, 2011 XXXth URSI
Conference_Location :
Istanbul
Print_ISBN :
978-1-4244-5117-3
Type :
conf
DOI :
10.1109/URSIGASS.2011.6051335
Filename :
6051335
Link To Document :
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