Senescence-Associated ß-Galactosidase as a Senescence Biomarker Showed in Rat Hippocampus

Author

Geng, Yq ; Guan, JT ; Wang, Bh ; Xu, Xh ; Fu, Yc

Author_Institution

Joint Shantou Int. Eye Center, Shantou Univ. Med. Coll., Shantou

Volume

1

fYear

2008

fDate

27-30 May 2008

Firstpage

173

Lastpage

177

Abstract

To investigated the activity ofSA-beta-GAL in rat hippocampus at different ages. Method: Hippocampus of 6, 18 and 24 month-age rats were observed by histochemical staining for SA-beta-GAL and cytochemical staining for SA-beta-GAL on prolong cultured hippocampal neurons. Results: SA-beta-GAL activity elevated in hippocampal pyramidal cells of CA3 region with advanced age. The upregulated SA-beta-GAL activity was accompanied by an increase prolong period of cultured hippocampal neurons. Interestingly, the staining of SA-beta-GAL was strikingly low in dentate gyrus, where the granules cells neurogenesis of rat from 6 to 18 and 24 month-age. Conclusion: These results suggested that SA-beta-GAL showed the senescent neurons in hippocampus, moreover, SA-beta-GAL is probably a potential tool to detect the neurogenesis pool in adult brain.

Keywords

brain; cellular biophysics; molecular biophysics; SA-beta-GAL; cultured hippocampal neurons; cytochemical staining; dentate gyrus; granules cells neurogenesis; hippocampal pyramidal cells; histochemical staining; rat hippocampus; senescence biomarker; senescence-associated beta-galactosidase; Aging; Biomarkers; Educational institutions; Forensics; Hippocampus; In vivo; Neurons; Rats; Scanning electron microscopy; Temperature; biomarker; hippocampus; rat; senescence associated ß-galactosidase;

fLanguage

English

Publisher

ieee

Conference_Titel

BioMedical Engineering and Informatics, 2008. BMEI 2008. International Conference on

Conference_Location

Sanya

Print_ISBN

978-0-7695-3118-2

Type

conf

DOI

10.1109/BMEI.2008.37

Filename

4548658