Title :
Imaging of Calcium Oscillation in Mouse Oocyte/zygote by Two Photon Laser Scanning Microscopy
Author :
Wang, Pengfei ; Fu, Jianhua ; Ma, Wanyun ; Chen, Dieyan ; Lv, Danyu ; Chen, Rui ; Bai, Wenjia
Author_Institution :
Dept. of Phys., Tsinghua Univ., Beijing
Abstract :
Calcium is one of the most important and versatile intracellular messenger, known as "the signal of life and death ". It has been found that, at certain time in the cell cycle, there is an oscillation of Ca2+ and its transmission (the Calcium wave) in the cell. Some useful information can be got from this phenomenon, like the speed of metabolism and the activity of the cell, ect. Using two photon laser scanning microscopy (TPLSM) system, we observed the dynamic process of the Ca2+ oscillation in mouse oocyte and zygote, obtaining the accurate cycle and amplitude of the oscillation. Then we successfully applied the results to the animal model experiment on hyperglycemia mice, with comparison to that on normal ones.
Keywords :
bio-optics; biological techniques; calcium; cellular biophysics; laser beam applications; optical microscopy; animal model experiment; calcium oscillation; cell cycle; hyperglycemia; intracellular messenger; metabolism; mouse; oocyte; two photon laser scanning microscopy; zygote; Animals; Biomedical imaging; Calcium; Embryo; Laboratories; Laser modes; Laser theory; Laser tuning; Mice; Microscopy;
Conference_Titel :
BioMedical Engineering and Informatics, 2008. BMEI 2008. International Conference on
Conference_Location :
Sanya
Print_ISBN :
978-0-7695-3118-2
DOI :
10.1109/BMEI.2008.144
