Imaging of Calcium Oscillation in Mouse Oocyte/zygote by Two Photon Laser Scanning Microscopy

Author

Wang, Pengfei ; Fu, Jianhua ; Ma, Wanyun ; Chen, Dieyan ; Lv, Danyu ; Chen, Rui ; Bai, Wenjia

Author_Institution

Dept. of Phys., Tsinghua Univ., Beijing

Volume

2

fYear

2008

fDate

27-30 May 2008

Firstpage

81

Lastpage

84

Abstract

Calcium is one of the most important and versatile intracellular messenger, known as "the signal of life and death ". It has been found that, at certain time in the cell cycle, there is an oscillation of Ca²⁺ and its transmission (the Calcium wave) in the cell. Some useful information can be got from this phenomenon, like the speed of metabolism and the activity of the cell, ect. Using two photon laser scanning microscopy (TPLSM) system, we observed the dynamic process of the Ca²⁺ oscillation in mouse oocyte and zygote, obtaining the accurate cycle and amplitude of the oscillation. Then we successfully applied the results to the animal model experiment on hyperglycemia mice, with comparison to that on normal ones.

Keywords

bio-optics; biological techniques; calcium; cellular biophysics; laser beam applications; optical microscopy; animal model experiment; calcium oscillation; cell cycle; hyperglycemia; intracellular messenger; metabolism; mouse; oocyte; two photon laser scanning microscopy; zygote; Animals; Biomedical imaging; Calcium; Embryo; Laboratories; Laser modes; Laser theory; Laser tuning; Mice; Microscopy;

fLanguage

English

Publisher

ieee

Conference_Titel

BioMedical Engineering and Informatics, 2008. BMEI 2008. International Conference on

Conference_Location

Sanya

Print_ISBN

978-0-7695-3118-2

Type

conf

DOI

10.1109/BMEI.2008.144

Filename

4549140