Automated and depth-controlled system for tissue dissection

In order to obtain sufficient quantity of pure populations of cells or a single cell from surrounding tissue for analytical investigation, many methods of micro-dissection have been proposed. This paper employs the stack type piezoelectric ceramics actuator for generating the ultrasonic vibration for dissection. And it presents a new method for acquiring the depth information that base on vision servo. Whilst, it can detect and track the position of needle tip in real time. Experiment results manifest the system can place the needle in any position above the organization and realize dissecting arbitrary shape in specify position; the target area selection is convenient and flexible; the target area can be removed when dissection is finished. It is obvious our dissecting method has advantage of improving the level of automation and efficiency.