A patch-clamp device with integrated actuators for cell selection and positioning

A patch-clamp (PC) microchip with integrated air-bubble actuators (AB actuators) for cell positioning is demonstrated, showing the promise for an automated PC microsystem. AB actuators are pneumatic actuators. The air pressure is generated by PDMS membrane mechanical deformation. By integrating with microfluidic channels and PC electrodes, AB actuators can potentially help avoid drawbacks of random cell selection or positioning for a PC microchip, paving the way for an automated PC microsystem. In this effort, the lateral electrode-pipette, located opposite to the outlet of an AB actuator, is designed for trapping a cell. The positioning and capturing of 3T3 cells were demonstrated successfully. Measurements found that the electrode impedance is 1 MOmega; the seal resistance is 5 MOmega with a captured cell. Optimization of the size and shape of the pipette is underway to enhance the seal resistance.