Low voltage plug dispersion compensation for moving field capillary electrophoresis with flow loss via the side channels

In this work, we present a novel technique to compensate the plug dispersion for the moving field capillary electrophoresis (CE) in the presence of flow loss via the side channels. A forward electroosmotic pump (EOP) is employed to compensate the back flow in the electroosmotic (EOF) segment between the active electrodes. By the analog electrical models to the fluid systems, the optimal pump structure is found out and proposed to reduce the required EOP voltage. To avoid the flow loss to the side channels, the slender channel clusters will be used to increase the flow resistance of the side channels. The finite element modeling is performed and the result, which is completely consistent with the analytical calculation result, shows that it can be a promising solution to the moving field CE.