Cell affinity of PHBV thin films evaluated by a parallel plate flow chamber

The aim of the study is to evaluate the cell affinity of poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV) thin films using a parallel plate flow chamber system. The PHBV thin films were modified by anhydrous ammonia gaseous plasma treatment. Mouse 3T3 fibroblast cells were seeded on bare and plasma modified PHBV thin films, respectively. A laminar flow enforces the cells on these films in a parallel plate flow chamber system. The percentage of initial cells remaining on the films after serials flows was used to evaluate the cell affinity of films. The results showed that, compared with control groups, ammonia plasma treatment could promote the cell affinity of PHBV films. It also indicated that the parallel plate flow chamber system can be used to evaluate the cell affinity of a material.