Title :
Functionalized bio-nanoparticles in immunosensing using Surface-Enhanced Raman scattering methods
Author :
Lin, Chi-Chang ; Yu, Chen-Yuan ; Yang, Ying-Mei ; Chen, Yan-Fu ; Chang, Hsien-Chang
Author_Institution :
Inst. of Biomed. Eng., Nat. Cheng Kung Univ., Tainan
Abstract :
An unique, sensitive, and highly specific immunoassay system using gold nanoparticles and surface-enhanced Raman spectroscopy (SERS) is described. To demonstrate the analytical capabilities of the new technique, Raman reporter (DTNB) that is coimmobilized with biospecific anti-protein A on gold nanoparticles (AuNP). Anti-protein A-AuNP-DTNB, which combing both electromagnetic field and chemical enhancement, was used to exploits the SERS-derived signal. A dynamic range of 2-3 orders of magnitude and 1-10 pg/mL of detection limitation of protein A were achieved. The results indicated this new technique could be used in clinical diagnostic applications with fast, high sensitivity and high-throughput screening of antibodies.
Keywords :
bio-optics; biomedical measurement; biosensors; gold; molecular biophysics; nanobiotechnology; nanoparticles; optical sensors; proteins; surface enhanced Raman scattering; SERS-derived signal; antibodies; biospecific antiprotein A; chemical enhancement; clinical diagnostic applications; electromagnetic field enhancement; functionalized bionanoparticles; gold nanoparticles; high-throughput screening; immunoassay system; immunosensing; surface-enhanced Raman scattering; Biomedical engineering; Glass; Gold; Immune system; Nanoparticles; Proteins; Raman scattering; Rough surfaces; Spectroscopy; Surface roughness; Surface-Enhanced Raman Spectroscopy (SERS); antibody; mercaptobenzoic; molecular probe; old nanoparticles; protein A;
Conference_Titel :
Nano/Micro Engineered and Molecular Systems, 2009. NEMS 2009. 4th IEEE International Conference on
Conference_Location :
Shenzhen
Print_ISBN :
978-1-4244-4629-2
Electronic_ISBN :
978-1-4244-4630-8
DOI :
10.1109/NEMS.2009.5068714
