A Portable Two-photon Fluorescence Microendoscope Based on a Two-dimensional Scanning Mirror

Author

Piyawattanametha, Wibool ; Cocker, Eric D. ; Barretto, Robert P J ; Jung, Juergen C. ; Flusberg, Benjamin A. ; Ra, Hyejun ; Solgaard, Olav ; Schnitzer, Mark J.

Author_Institution

Stanford Univ., Stanford

fYear

2007

fDate

Aug. 12 2007-July 16 2007

Firstpage

6

Lastpage

7

Abstract

Towards overcoming the size limitations of conventional two-photon fluorescence microscopy for brain imaging in freely moving mice, we introduce a portable laser-scanning microendoscope based on a microelectromechanical systems (MEMS) two-dimensional (2-D) scanning mirror, compound gradient refractive index (GRIN) micro-lenses, and a photonic bandgap fiber (PBF). The microendoscope achieves fast line scanning acquisition rates up to 3.5 kHz and micron-scale imaging resolution.

Keywords

biomedical optical imaging; brain; endoscopes; fibre optic sensors; gradient index optics; image resolution; laser applications in medicine; micro-optomechanical devices; microlenses; micromirrors; neurophysiology; optical microscopy; photonic band gap; two-photon processes; brain imaging; compound gradient refractive index; frequency 3.5 kHz; laser-scanning microendoscope; mice; microelectromechanical systems; microlenses; micron-scale imaging resolution; photonic bandgap fiber; portable two-photon fluorescence microendoscope; scanning acquisition rates; two-dimensional scanning mirror; two-photon fluorescence microscopy; Brain; Fiber lasers; Fluorescence; Mice; Microelectromechanical systems; Micromechanical devices; Microscopy; Mirrors; Refractive index; Two dimensional displays; 3-D image; fluorescence image; scanner; scanning mirror; two-photon;

fLanguage

English

Publisher

ieee

Conference_Titel

Optical MEMS and Nanophotonics, 2007 IEEE/LEOS International Conference on

Conference_Location

Hualien

Print_ISBN

978-1-4244-0641-8

Type

conf

DOI

10.1109/OMEMS.2007.4373812

Filename

4373812