Title :
Real-time label-free detection of DNA synthesis by FBAR-based mass sensing
Author :
Lin, Anderson ; Sahin, Furkan E. ; Chen, Shih-Jui ; Pham, Phuong ; Kim, Eun Sok
Author_Institution :
Dept. of Electr. Eng.-Electrophys., Univ. of Southern, CA, USA
Abstract :
This paper reports a real-time detection of DNA polymerase reaction through mass sensing with film bulk acoustic resonator (FBAR) coated with self-assembled DNA sequences. This label-free, real-time detection of DNA synthesis can potentially lead to detection of single nucleotide addition for DNA sequencing application, which can be developed into a low-cost, third-generation DNA sequencing system capable of reading thousands of nucleotides in a single run.
Keywords :
DNA; acoustic resonators; biomedical ultrasonics; bulk acoustic wave devices; self-assembly; DNA polymerase reaction; DNA synthesis; FBAR-based mass sensing; film bulk acoustic resonator; real-time label-free detection; self-assembled DNA sequences; single nucleotide addition; DNA; Electrodes; Film bulk acoustic resonators; Frequency measurement; Gold; Polymers; Resonant frequency;
Conference_Titel :
Ultrasonics Symposium (IUS), 2010 IEEE
Conference_Location :
San Diego, CA
Print_ISBN :
978-1-4577-0382-9
DOI :
10.1109/ULTSYM.2010.5935968
