Preparation of the glucose sensor based on nano-au modified electrode

In recent years, the nano-materials, which have advantages of small particle size, large specific surface area, high surface reactive activity, good catalytic activity and strong adsorptivity, have attracted considerable attention. In this paper, the glucose non-enzymatic biosensor based on nano-Au has been fabricated by self-assembly technology. Firstly, the gold colloid were prepared according to the follow steps: With string, 100 ml of 0.01% HAuCl₄ aqueous solution was heated to a boiling condition, then 2 ml of 1% trisodium citrate was rapidly added. After boiling, keeping string for 15 min, the color of the mixture changed from pale yellow to blue, and finally arrived at ruby-red Then a carefully cleaned Ag electrode was dipped into the gold colloid to obtain the non-enzymatic glucose sensor. Their electrochemical properties were investigated The senses have been successfully applied for the determination of glucose and the result also showed that the non enzymatic glucose sensor had good current response to glucose in alkaline medium. Under optimal conditions, the developed glucose biosensor exhibited fast response to glucose and linear ranges were 0.2 to 175.2 μmol/L, with a detection limit of 0.15 μmol/L. In addition, the as-prepared biosensor exhibiter superior stability high sensitivity and good selectivity.