Title :
Regulation and role of water channel protein, aquaporin in the brain
Author_Institution :
Dept. of Biol. Sci., Ulsan Univ., South Korea
fDate :
26 June-2 July 2005
Abstract :
In the BAS8.1 astroglioma cell lines, TTF-1 transactivated the AQP-1 promoter in a dose-dependent manner. Electrophoretic mobility shift assays showed that TTF-1 binds to specific subsets of its consensus DNA binding sites in the AQP-1 promoter. Assays using cells treated with a high concentration of different solutes revealed a decrease in luciferase activities both of the TTF-1 and AQP-1 promoters. Inhibition of TTF-1 synthesis by antisense oligodeoxynucleotide (ODN) injection decreases AQP-1 synthesis and leads to a decrease in CSF formation. These data suggest that TTF-1 plays an important regulatory role in the transcription of AQP-1, which may be important for formation of cerebrospinal fluid.
Keywords :
DNA; brain; cellular biophysics; molecular biophysics; proteins; water; AQP-1 promoter; AQP-1 transcription; BAS8.1 astroglioma cell lines; CSF formation; DNA binding; ODN injection; TTF-1 binding; antisense oligodeoxynucleotide; aquaporin; brain; cerebrospinal fluid formation; electrophoretic mobility shift assay; luciferase activity; water channel protein; Biology; Biomembranes; Cells (biology); Central nervous system; DNA; Humans; Liver; Lungs; Permeability; Proteins;
Conference_Titel :
Science and Technology, 2005. KORUS 2005. Proceedings. The 9th Russian-Korean International Symposium on
Print_ISBN :
0-7803-8943-3
DOI :
10.1109/KORUS.2005.1507662