Title :
Convection compensated electrophoretic NMR (CC-ENMR) for structure characterization of mixed proteins in biological buffer solutions
Author :
He, Qiuhong ; Wei, Zhaohui
Author_Institution :
Dept. of Chem., Connecticut Univ., Storrs, CT, USA
Abstract :
A novel ENMR method-Convection Compensation ENMR (CC-ENMR)-has been developed, which permits selective detection of electrophoretic motion of ionic species. The convection generated ENMR spectral artifacts were removed by gradient moment nulling technique, whereas polarity switching of the applied DC electric field canceled out the flow compensation effect for ionic movement driven by the electric field
Keywords :
biological NMR; electrophoresis; molecular biophysics; molecular configurations; proteins; spin echo (NMR); applied DC electric field; biological buffer solutions; convection compensated; electrophoretic NMR; electrophoretic motion; gradient moment nulling technique; ionic species; mixed proteins; polarity switching; protein conformations; protein interactions; selective detection; spectral artifacts removal; spin echo; structure characterization; Amino acids; Chemistry; DC generators; Fluid flow measurement; Frequency modulation; Helium; Motion detection; Multidimensional systems; Nuclear magnetic resonance; Proteins;
Conference_Titel :
Bioengineering Conference, 2000. Proceedings of the IEEE 26th Annual Northeast
Conference_Location :
Storrs, CT
Print_ISBN :
0-7803-6341-8
DOI :
10.1109/NEBC.2000.842389
