Convection compensated electrophoretic NMR (CC-ENMR) for structure characterization of mixed proteins in biological buffer solutions

Author

He, Qiuhong ; Wei, Zhaohui

Author_Institution

Dept. of Chem., Connecticut Univ., Storrs, CT, USA

fYear

2000

fDate

2000

Firstpage

81

Lastpage

82

Abstract

A novel ENMR method-Convection Compensation ENMR (CC-ENMR)-has been developed, which permits selective detection of electrophoretic motion of ionic species. The convection generated ENMR spectral artifacts were removed by gradient moment nulling technique, whereas polarity switching of the applied DC electric field canceled out the flow compensation effect for ionic movement driven by the electric field

Keywords

biological NMR; electrophoresis; molecular biophysics; molecular configurations; proteins; spin echo (NMR); applied DC electric field; biological buffer solutions; convection compensated; electrophoretic NMR; electrophoretic motion; gradient moment nulling technique; ionic species; mixed proteins; polarity switching; protein conformations; protein interactions; selective detection; spectral artifacts removal; spin echo; structure characterization; Amino acids; Chemistry; DC generators; Fluid flow measurement; Frequency modulation; Helium; Motion detection; Multidimensional systems; Nuclear magnetic resonance; Proteins;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioengineering Conference, 2000. Proceedings of the IEEE 26th Annual Northeast

Conference_Location

Storrs, CT

Print_ISBN

0-7803-6341-8

Type

conf

DOI

10.1109/NEBC.2000.842389

Filename

842389