Title :
Direct bio-mechanical sensing of enzymatic reaction On DNA by silicon nanotweezers
Author :
Kumemura, M. ; Collard, D. ; Yoshizawa, S. ; Fourmy, D. ; Lafitte, N. ; Jalabert, L. ; Takeuchi, S. ; Fujii, T. ; Fujita, H.
Author_Institution :
LIMMS-IIS, Univ. of Tokyo, Tokyo, Japan
Abstract :
This paper demonstrates the real-time sensing of enzymatic reactions on a DNA bundle trapped by silicon nanotweezers. The digestion of DNA in protein solution (Hind III) is monitored by tracking the change in frequency response of the immersed tweezers. This new direct biomechanical detection clears the way for systematic bio tests at the molecular level by an integrated MEMS device.
Keywords :
bioMEMS; biochemistry; biomechanics; biosensors; enzymes; molecular biophysics; nanobiotechnology; silicon; DNA bundle; bio-mechanical sensing; biomechanical detection; enzymatic reaction; immersed tweezers; integrated MEMS device; molecular level; protein solution; silicon nanotweezers; systematic bio tests; Arm; Atomic force microscopy; Biochemistry; DNA; Frequency response; Micromechanical devices; Molecular biophysics; Nanobioscience; Probes; Silicon;
Conference_Titel :
Micro Electro Mechanical Systems (MEMS), 2010 IEEE 23rd International Conference on
Conference_Location :
Wanchai, Hong Kong
Print_ISBN :
978-1-4244-5761-8
Electronic_ISBN :
1084-6999
DOI :
10.1109/MEMSYS.2010.5442356
